Abstract
Reducing the amounts consumed of organic solvents while keeping good chromatographic performance has been a significant step towards the greening of analytical methodologies. When sodium dodecyl sulfate (SDS) and Brij-35 surfactants are combined in a mobile phase, they can be used as a green alternative to organic modifiers. Surfactants have numerous advantages, including low cost and toxicity, safe environmental disposal, and unique selectivity, in addition to high solubilization capabilities. In this research, two highly selective chromatographic methods were adopted for the determination of betahistine (BHS) in the presence of its pharmacopeial impurity 2-(2-hydroxyethyl)pyridine (HEP). A solvent-free HPLC method was validated, in which the mixture was separated using a C18 column (3.5 µm, 75.0 × 4.6 mm) and a mobile phase composed of 0.01 M Brij-35, 0.12 M SDS, and 0.02 M disodium hydrogen phosphate adjusted to a pH of 5.5 using phosphoric acid. The flow rate was 1.5 mL min−1 and the resolved peaks were detected at 260 nm. Another HPTLC-densitometric method was validated using HPTLC aluminum plates coated with silica gel 60 F254 as the stationary phase and a developing system consisting of methylene chloride/methanol/ethyl acetate/ammonia (at a ratio of 5:2:2:0.2 by volume); the separated bands were scanned at 260 nm.
Highlights
Menière’s disease (MD) was named after Prosper Menière, a French physician who proposed in 1861 that episodic vertigo and hearing loss could be caused by an inner ear problem [1]
The analytes were optimally separated with HPLC by isocratic elution using a mobile phase of 0.01 M of Brij-35, 0.12 M of sodium dodecyl sulfate (SDS), and 0.02 M of disodium hydrogen phosphate adjusted to a pH of 5.5 with phosphoric acid
Development was performed at room temperature inside a twin-trough securely closed TLC jar, which was saturated by the mobile phase for 30 min before chromatogram development
Summary
Menière’s disease (MD) was named after Prosper Menière, a French physician who proposed in 1861 that episodic vertigo and hearing loss could be caused by an inner ear problem [1]. Menière’s disease is characterized by tinnitus, vertigo, and hearing loss. There is currently no cure for Menière’s disease, and the cause of the condition is still unknown. MD is a clinical illness that affects 50 to 200 persons per 100,000, with the majority of cases occurring between the ages of 40 and 60. Separations 2022, 9, 49 per 100,000, with the majority of cases occurring between the ages of 40 and 60. CPoynrsied-ine queanncdesitisndcleurdiveaitrivrietsathioanveobf ethene sliknikne, deyteosb, notohsea,caunted athnrdolaotn; glo-ntegr-mterhmeaclothmrpislkicsa.tAiocnustienc-onclusdeeqfueetanlcdesaminacglued. He ierpraittaottioxnicoiftythaendsknine,pehyreosto, nxiocsitey, ahnadvetharlsooatb; eloengli-ntekremd tcoompyprliidcaintieons andinictsluddeerifveatatilvdeasm. FFuurrtthheerrmmoorree,, tthhee pprrooppoosseedd mmeetthh-ods odsaraerevevreyrysesnensistiitviveetotoaacccucuraratetelylyaasssesessssHHEEPP(u(upptoto00.0.055%%foforrHHPPLLCCaannddaabboouutt00.1.155%%for forHHPPTTLLCC)),,wwhhiicchh iiss ccoonnssiisstteenntt wwiitthh tthhee iimmppuurriittyy pprroofifillee rreeqquuiirreemmeennttss ((mmaaxxiimmuumm lliimmiitt of of 00..22%%)) ggiivveennbbyytthhee BBPP22001177 [[77]] aanndd UUSSPP 4433 [[88]]
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