Echinococcus granulosus experimental infection down-modulates Nitric Oxide production in DSS induced colitis in mice.

Abstract

Event Abstract Back to Event Echinococcus granulosus experimental infection down-modulates Nitric Oxide production in DSS induced colitis in mice. Lila Khelifi1*, Moussa Labsi1*, Imen Soufli1 and Chafia Touil-Boukoffa1* 1 University of Sciences and Technology Houari Boumediene, Laboratory of Cellular and Molecular Biology, Algeria Background: Inflammatory bowel diseases (IBD) are immune-mediated diseases, which are common in western industrialized countries while they are uncommon in developing countries. The hygiene hypothesis suggests that the loss of exposure to helminthes such as Echinococcus granulosus engendered a development of IBD. This parasite causes cystic hydatid disease which is endemic in Algeria. Previous data reported that helminthes reduce inflammatory process in experimental colitis. The aim of our study is to investigate the immuno-modulatory effect of the secondary infection with Echinococcus granulosus on Nitric Oxide (NO) production in Dextran Sulfate Sodium (DSS) induced colitis model. Materials and Methods: A group of Swiss mice is infected intraperitoneally (i.p) with 200 µl of a suspension containing 2000 viable protoscoleces of Echinococcus granulosus. After 3 months of infection, experimental colitis is induced in infected and uninfected mice by dissolving DSS in their drinking water for 7 days and then were sacrificed. We estimated disease activity index (DAI) which took into account body weight, stool consistency and gross bleeding. Nitric oxide (NO), is determined to investigate the inflammatory response. Results: Our results show that the disease activity index (DAI) of colitis increased at slow pace in the infected mice which developed cysts in comparison to the uninfected mice. In addition, a significant decrease in nitric oxide production is observed in plasma of infected mice. Conclusion: Our experiments suggest a potential immuno-modulatory effect of Echinococcus granulosus on Dextran Sulfate Sodium (DSS) experimental colitis by nitric oxide (NO) down-modulation. Acknowledgements The authors thank the technical and surgical staff of the department of thoracic surgery, Mustapha-Bacha Hospital of Algiers for providing cyst samples Keywords: Dextran Sulfate Sodium (DSS), Echinococcus granulosus, experimental colitis, helminthes, Hygiene Hypothesis, inflammatory bowel diseases (IBD), Nitric Oxide (NO). Conference: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014), Tlemcen, Algeria, 17 Oct - 20 Oct, 2014. Presentation Type: Poster Presentation Topic: Mucosal immunity Citation: Khelifi L, Labsi M, Soufli I and Touil-Boukoffa C (2014). Echinococcus granulosus experimental infection down-modulates Nitric Oxide production in DSS induced colitis in mice.. Front. Immunol. Conference Abstract: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014). doi: 10.3389/conf.fimmu.2014.04.00015 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 Sep 2014; Published Online: 01 Dec 2014. * Correspondence: Ms. Lila Khelifi, University of Sciences and Technology Houari Boumediene, Laboratory of Cellular and Molecular Biology, bab-ezzouar, Algiers, 16000, Algeria, freelita@yahoo.fr Mr. Moussa Labsi, University of Sciences and Technology Houari Boumediene, Laboratory of Cellular and Molecular Biology, bab-ezzouar, Algiers, 16000, Algeria, moussalabsi@yahoo.fr Prof. Chafia Touil-Boukoffa, University of Sciences and Technology Houari Boumediene, Laboratory of Cellular and Molecular Biology, bab-ezzouar, Algiers, 16000, Algeria, touilboukoffa@yahoo.fr Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Lila Khelifi Moussa Labsi Imen Soufli Chafia Touil-Boukoffa Google Lila Khelifi Moussa Labsi Imen Soufli Chafia Touil-Boukoffa Google Scholar Lila Khelifi Moussa Labsi Imen Soufli Chafia Touil-Boukoffa PubMed Lila Khelifi Moussa Labsi Imen Soufli Chafia Touil-Boukoffa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.