Abstract
ABSTRACT. The difference in ecdysteroid activity in short‐day (10‐h photo‐phase) and long‐day (16‐h photophase) Leptinotarsa decemlineata Say adults was characterized by high performance liquid chromatography, which revealed that for short‐day beetles ecdysterone and ecdysone are the major constituents of the free ecdysteroids, whereas in long‐day beetles products co‐migrating with 2‐deoxyecdysone and 2‐deoxyecdysterone are just as abundant. Ecdysteroids were detected (15 ng/ml) only in the haemolymph of females. Ligation experiments showed that the induction of the diapause syndrome by inactivation of the corpora allata or the brain not only resulted in the formation of the typical diapause proteins but also in an increase of the ecdysteroid activity. It is suggested that adult diapause in L.decemlineata is regulated by a multifactorial system.
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