Eccentric Exercise Decrease Blood Glucose Level and Improve Protein Level of Glucose Transporters in Diabetic Mice Muscle

Abstract

Background: Previously, eccentric exercise improved on blood glucose level better than concentric andisometric exercise. The improvement of blood glucose level was resulted from higher glucose uptakeactivity, particularly at the muscles. Unfortunately here isn’t yet information whether it is facilitated byglucose transporter type 1 or glucose transporter type 4.Objectives: The aim of the present study was to investigate the effect of eccentric activity on glucosetransporter type 1 and glucose transporter type 4 in gastrocnemius muscle of streptozotocin-induced diabetesmellitus mice.Methods: Diabetic mice were grouped randomly into 4 groups (7 mice each group). Two groups wereobserved during fasting and others were observed during post prandial. Single bout of eccentric activity wasgiven by downhill running on 10o degree decline treadmill. The protein level of glucose transporters weremeasured using monoclonal antibody in ELISA protocol. Blood glucose level was measured on fasting andpost prandial using colorimetric protocol of EASY TOUCH app.Result: Glucose levels (fasting and post prandial) of eccentric group were significantly lower than isometricgroup. Consistently, protein level of glucose transporters (type 1 and type 4) were significantly higher thanisometric group. Type-4 glucose transporter level was higher than type-1 glucose transporter, found in calfmuscle.Conclusions: single bout of eccentric exercise on downhill running improve blood glucose level due to bothGLUT-1 and GLUT-4 protein levels improvement.