EasySep&[trade] T75 Magnet: A Novel Magnetic Platform for Large-Volume Cell Isolation from Whole Blood and Leukapheresis Packs

Abstract

Abstract Isolating cells from large volume-samples such as leukapheresis or whole blood bags is a common procedure that precedes many immunological studies. Working with large volumes can be challenging as the sample often needs to be split and processed in parallel, which can be tedious and may delay downstream studies. The EasySep™ T75 Magnet simplifies cell separation procedures when processing up to 225 mL of leukapheresis sample or 125 mL of whole blood, and is based on column-free immunomagnetic EasySep™ cell isolation technology. Negative selection protocols have been optimized for isolation of human T cells (purity: 95.3 ± 2.5%, n=5), CD4+ T cells (purity: 97.2 ± 1.4%, n=4), CD8+ T cells (purity: 91.9 ± 2.2%, n=5), and monocytes (purity: 91.5 ± 1.0%, n=3). Positive selection protocols have been optimized for isolation of CD3+ cells (purity: 95.4 ± 2.9%, n=9), CD4+ cells (purity: 88.8 ± 3.2%, n=3), CD8+ cells (purity: 95.1 ± 2.4%, n=6), and CD14+ cells (purity: 96.2 ± 0.7%, n=3). Protocols for depletion of red blood cells (RBCs) (residual RBCs: 2.5 ± 1.7%, n=5) and enrichment of peripheral blood mononuclear cells from whole blood (purity: 99.2 ± 0.5%, n=5) have also been developed. Notably, these protocols can be completed in 20 – 26 minutes, making the EasySep™ T75 Magnet the fastest and simplest method for isolating highly purified immune cells from large-volume whole blood and leukapheresis samples.