Abstract
During the 2019 Eastern equine encephalitis virus (EEEV) outbreak in Michigan, two 2-month old Mexican wolf pups experienced neurologic signs, lymphohistiocytic neutrophilic meningoencephalitis with neuronal necrosis and neuronophagia, and acute death. We identified EEEV by reverse transcription real-time PCR and in situ hybridization. Vector mosquitoes were trapped at the zoo.
Highlights
During the 2019 Eastern equine encephalitis virus (EEEV) outbreak in Michigan, two 2-month old Mexican wolf pups experienced neurologic signs, lymphohistiocytic neutrophilic meningoencephalitis with neuronal necrosis and neuronophagia, and acute death
In the midst of the outbreak, EEEV was diagnosed in two 2-month old Mexican wolf (Canis lupus baileyi) pups at Binder Park Zoo (BPZ)
We assumed that transmission of EEEV to the wolf pups was from a mosquito bite that occurred at their exhibit location
Summary
During the 2019 Eastern equine encephalitis virus (EEEV) outbreak in Michigan, two 2-month old Mexican wolf pups experienced neurologic signs, lymphohistiocytic neutrophilic meningoencephalitis with neuronal necrosis and neuronophagia, and acute death. Additional reports exist of clinical disease in a wide variety of mammalian and avian species, including swine, cattle, white-tailed deer, alpacas, seals, domestic canids, pheasants, emus, penguins, and cassowary birds [5,6,7,8,9,10]. Author affiliations: Binder Park Zoo, Battle Creek, Michigan, USA (K.A. Thompson); Michigan State University Veterinary Diagnostic Laboratory, Lansing, Michigan, USA
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