Abstract
Objective: MiRNAs are important regulators of translation and have been described as biomarkers of a number of cardiovascular diseases, including stroke. The purpose of the study was to determine expression levels of serum miR-1297 in patients with aneurysmal subarachnoid hemorrhage (aSAH), and to assess whether miR-1297 was the prognostic indicator of aSAH. Methods: We treated 128 aSAH patients with endovascular coiling. The World Federation of Neurological Surgeons (WFNS) grades, Hunt–Hess grades, and modified Fisher scores were used to assess aSAH severity. Neurologic outcome was assessed using the Modified Rankin Scale (mRS) at 1-year post-aSAH. Serum was taken at various time points (24, 72, and 168 h, and 14 days). Serum samples from aSAH patients and healthy controls were subjected to reverse transcription (RT) quantitative real-time PCR (RT-qPCR). Results: A poor outcome at 1 year was associated with significantly higher levels of miR-1297 value at the four time points, higher WFNS grade, higher Hunt–Hess grade, and higher Fisher score. Serum miR-1297 levels were significantly higher in patients, compared with healthy controls. There were significant correlations of miR-1297 concentrations in serum with severity in aSAH. The AUCs of miR-1297 at the four time points for distinguishing the aSAH patients from healthy controls were 0.80, 0.94, 0.77, and 0.59, respectively. After multivariate logistic regression analysis, only miR-1297 at 24 and 72 h enabled prediction of neurological outcome at 1 year. Conclusion: Serum was an independent predictive factor of poor outcome at 1 year following aSAH. This result supports the use of miR-1297 in aSAH to aid determination of prognosis.
Highlights
Aneurysmal subarachnoid hemorrhage is a serious, life-threatening type of stroke, characterized by bleeding into the meningeal subarachnoid space surrounding the brain
One of the major determinants of the neurological status after aneurysmal subarachnoid hemorrhage (aSAH) are characteristics of the initial hemorrhage during the hospital course, those related to early brain injury (EBI) and early cerebral vasospasms (CVs) [2]
The sensitivity and specificity at cut-off value of 7.97 were 87.50 and 90%, respectively. These findings suggest that serum miR-1297 had high power to distinguish aSAH patients from healthy controls, especially at 72 h post-aSAH
Summary
Aneurysmal subarachnoid hemorrhage (aSAH) is a serious, life-threatening type of stroke, characterized by bleeding into the meningeal subarachnoid space surrounding the brain. It is associated with approximately 50% mortality, with 10% of patients dying prior to reaching the hospital, and up to 45% dying within 30 days [1]. One of the major determinants of the neurological status after aSAH are characteristics of the initial hemorrhage during the hospital course, those related to early brain injury (EBI) and early cerebral vasospasms (CVs) [2]. Clinical trials have demonstrated c 2018 The Author(s).
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