Abstract
Influenza virus RNA labeled with 32PO 4 was annealed to viral complementary (messenger) RNA (cRNA) isolated from polysomes of infected cells. After purification, the hybrid molecules were examined by polyacrylamide-gel electrophoresis. As early as 20 min after infection, all eight segments of virus-specific cRNA were associated with polysomes, indicating a lack of temporal control at the transcriptional level. Also, it was found that the addition of actinomycin D to cells 2 hr after infection stopped further cRNA synthesis but had no effect on preexisting cRNA.
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