Abstract
Alzheimer Disease (AD) is a pathology suffered by millions of people worldwide and it has a great social and economic impact. Previous studies reported a relationship between alterations in different amino acids and derivatives involved in neurotransmission systems and cognitive impairment. Therefore, in this study the neurotransmission impairment associated to early AD has been evaluated. For this purpose, different amino acids and derivatives were determined in saliva samples from AD patients and healthy subjects, by means of an analytical method based on chromatography coupled to tandem mass spectrometry. Results showed statistically significant differences in salivary levels for the compounds myo-inositol, creatine and acetylcholine; and other compounds (myo-inositol, glutamine, creatine, acetylcholine) showed significant correlations with some cognitive tests scores. Therefore, these compounds were included in a multivariate analysis and the corresponding diagnosis model showed promising indices (AUC 0.806, sensitivity 61%, specificity 92%). In conclusion, some amino acids and derivatives involved in neurotransmission impairment could be potential biomarkers in early and non-invasive AD detection.
Highlights
Alzheimer Disease (AD) is a pathology suffered by millions of people worldwide and the increasing number of cases in recent years generates a great concern for the economic and social effects p roduced[1,2]
The accuracy of the method was evaluated by analysing spiked saliva samples containing analytes at different concentrations within the tested concentration ranges
The analytes stability was assayed after three freeze thaw cycles, determining the concentrations in spiked saliva samples (1000 nmol L-1, each analyte) by triplicate
Summary
Alzheimer Disease (AD) is a pathology suffered by millions of people worldwide and the increasing number of cases in recent years generates a great concern for the economic and social effects p roduced[1,2]. A reduction of synapsis o ccurs[13], and neurotransmission is altered In this sense, the study of different compounds involved in neurotransmission systems could be u seful[14]. Different amino acids such as glutamate, serine and alanine seem to play an important role in AD cognition decline[25]. This is the first study that evaluate the salivary levels of different related-neurotransmission compounds in AD patients. The aim of this study is to evaluate some amino acids and derivatives related to neurotransmission alterations under AD conditions as potential biomarkers for early and non-invasive AD detection
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
