Early Low-Fluence Red Light or Darkness Modulates the Shoot Regeneration Capacity of Excised Arabidopsis Roots.

Xi Wei,Ye Wang,Yanpeng Ding,Fuguang Li,Xiaoyang Ge

doi:10.3390/plants9101378

Xi Wei, Ye Wang + Show 3 more

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https://doi.org/10.3390/plants9101378

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Abstract

In plants, light is an important environmental signal that induces meristem development and interacts with endogenous signals, including hormones. We found that treatment with 24 h of low-fluence red light (24 h R) or 24 h of darkness (24 h D) following root excision greatly increased the frequency of shoot generation, while continuous low-fluence red light in callus and shoot induction stages blocked the explants’ ability to generate shoots. Shoot generation ability was closely associated with WUS expression and distribution pattern. 1-N-naphthylphtalamic acid (NPA) disrupted the dynamic distribution of the WUS signal induced by early 24 h R treatment, and NPA plus 24 R treatment increased the average shoot number compared with early 24 h R alone. Transcriptome analysis revealed that differentially expressed genes involved in meristem development and hormone signal pathways were significantly enriched during 24 R or 24 D induced shoot regeneration, where early 24 h R or 24 h D treatment upregulated expression of WOX5, LBD16, LBD18 and PLT3 to promote callus initiation and formation of root primordia, and also activated WUS, STM, CUC1 and CUC2 expression, leading to initiation of the shoot apical meristem (SAM). This finding demonstrates that early exposure of explants to transient low-fluence red light or darkness modulates the expression of marker genes related with callus development and shoot regeneration, and dynamic distribution of WUS, leading to an increased ability to generate shoots.

Highlights

Plant cells are pluripotent, meaning they have the potential to develop into an entire plant body from highly differentiated tissues or organs, or from a single somatic cell [1]
We found that exposure of explants to long-term low-fluence red light strongly inhibited the generation of adventitious shoots, while 24 h exposure to low-fluence red light after root excision significantly improved the efficiency of shoot regeneration
Root explants from wild-type Arabidopsis Col-0 were used to evaluate the effects of different combinations of light on the capacity for shoot regeneration, through treatments applied during the callus-induction medium (CIM) and shoot-induction medium (SIM) stages (Figure 1A)

Summary

Introduction

Plant cells are pluripotent, meaning they have the potential to develop into an entire plant body from highly differentiated tissues or organs, or from a single somatic cell [1]. Somatic organogenesis is important for transgenic plant generation [4,5]; shoot regeneration can be induced from callus tissues culture in two phases. Explants of excised Arabidopsis root or cotyledon are cultured on a callus-induction medium (CIM) under dark conditions to induce callus formation [5]. Callus cells form when the plant tissue becomes dedifferentiated and acquires pluripotency, which is necessary for shoot regeneration [6,7]. Some studies have shown that callus initiation on a CIM is similar to the rooting pathway in non-root organs where the newly formed callus resembles a group of root primordium-like cells [8,9].

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