Abstract
Abstract The early stages of infection of insect blood cells by Sericesthis iridescent virus (SIV) were studied. Blood cells of Galleria mellonella (L.) infected with SIV at 22° were sampled at different intervals and then subjected to electron microscopy and high-resolution autoradiography. At 2 hours post inoculation virus was found inside the cells within phagocytic vesicles. A large proportion of virus had disappeared after all incubation period of 3 hours. Viral DNA synthesis in the cytoplasm was demonstrated as early as 4Y2 hours post SR' inoculation by using tritiated thymidine and autoradiography. Earliest occurrence of progeny virus within foci was found to be at 12 hours. Decrease in nuclear incorporation of thymidine 3H indicates all interference of normal nuclear DNA synthesis in the course of SIV infection.
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