Abstract
The surfaces of ovine thyroid cells in monolayer culture respond characteristically to the addition of thyrotropin (TSH) to the medium. Pseudopodia and microvilli appear within minutes of TSH addition and the cell surface assumes a rough texture. The response is maximal between 15 and 30 min after stimulation. This action of TSH is mimicked by N6, O2-dibutyryl adenosine 3′,5′-cyclic monophosphate and by prostaglandin E1 and to a lesser degree by cyclic AMP. TSH action is not mimicked by cyclic GMP, dibutyryl cyclic GMP, or by the protein hormones: human chorionic gonadotropin, ACTH, insulin, or prolactin. Surface activity, including bleb formation which is maximal at Day 2, has subsided by the fourth day after TSH addition and the surface appears smooth and flat, thus resembling nonstimulated cultures. The response to thyrotropin is greatly diminished in the presence of Li+, cycloheximide, actinomycin D, vinblastine, and cytochalasin B. The cell surface response of thyroid cells in culture is consistent with the acute responses of the intact gland to TSH such as endocytosis and colloid droplet formation.
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