Early Effects of Neonatal Rat Desympathization on Secondary Heart Rhythms

Abstract

Neonatal Wistar rats for the first 3 weeks of life were injected intraperitoneally with isobarine every other day. The single doze was 40 mg/kg. Control animals were injected with saline. Degenerative changes in sympathetic ganglia were evident as early as in the 10-day old animals and increased by 18–19 days. The heart rate in the desympathized animals was lower than in control from 10–11 to 18–19 days, but by the end of the 3rd week the differences were eliminated. The same occurred with respiration rate. At same terms there was an essential decrease of amplitude of the heart rate high-frequency fluctuations synchronous with respiration and of the periodogram slow waves with the period about 1 min. Using the method of fast Fourier transform, the power spectra of heart rate fluctuations (secondary heart rhythms) in 5 frequency ranges (0–0.01, 0.01–0.03, 0.03–0.1, 0.1–1.0, and 1.0–2.5 Hz) were calculated. Desympathization leads to a decrease of the fluctuation power in all ranges, but in the ultralow-frequency range this decrease is the least pronounced, which suggests the presence of non- sympathetic mechanisms in their genesis. The greatest changes occur in the middle-frequency area. In all cases, differences from control values increase from the 10–11th to the 18–19th days, after which a tendency for restoration is observed, in spite of an enhancement of processes of degeneration of sympathetic neurons. This indicates an activation of the compensatory mechanisms, due to which consequences of desympathization are partially smoothed at distant terms of studies.