Abstract
Materials and methods From January to December 2008, 114 infants born to HIV-1 infected mothers were referred to the University Hospital Yalgado Ouedraogo (Ouagadougou, Burkina Faso), for early diagnosis of HIV infection. Two to 3 mL of blood sample were collected on EDTA/K3 microtubes from each infant. After centrifugation, the plasma samples were stored at -80°C, until their use. HIV-1 RNA was detected in each sample using Abbott RealTime HIV-1 Assay (Abbott Molecular) and m2000rt protocol: the RNA detection threshold was 40 copies/mL (1.6 Log). From each infant with RNA positive result, a second plasma sample was collected 4 weeks later to confirm the previous RNA result Sociodemographic data were collected from the infants and analysed.
Highlights
Hermann Somlare*1,2, Lassana Sangare1,3, Saydou Yamegogo1,2, Carine Ouedraogo2, Yolande Dembel1, Monique Soro2, Parfait Some4 and Caroline Yonaba4
From January to December 2008, 114 infants born to HIV-1 infected mothers were referred to the University Hospital Yalgado Ouédraogo (Ouagadougou, Burkina Faso), for early diagnosis of HIV infection
The plasma samples were stored at -80°C, until their use
Summary
Hermann Somlare*1,2, Lassana Sangare, Saydou Yamegogo, Carine Ouedraogo, Yolande Dembel, Monique Soro, Parfait Some and Caroline Yonaba. Address: 1Laboratory of Bacteriology and Virology of Hospital University Center Yalgado Ouédraogo, West Africa, Burkina Faso, 2UFR Sciences de la Vie et de la Terre of Unviersity of Ouagadougou, West Africa, Burkina Faso, 3UFR Sciences de la Santé, West Africa, Burkina Faso and 4Paediatric clinic of Hospital University Center Yalgado Ouédraogo, West Africa, Burkina Faso. Published: 22 July 2009 Retrovirology 2009, 6(Suppl 1):P10 doi:10.1186/1742-4690-6-S1-P10. Fifth Dominique Dormont International Conference. Host-Pathogen Interactions in Chronic Infections Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here. http://www.biomedcentral.com/content/pdf/1742-4690-6-S1-info.pdf
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