Early development of medaka Oryzias latipes muscles as revealed by transgenic approaches using embryonic and larval types of myosin heavy chain genes

Abstract

We cloned three full-length cDNAs encoding myosin heavy chains (MYHs) previously found to be expressed in embryos or larvae of medaka Oryzias latipes. Based on cDNA sequence information, the three medaka MYH genes, mMYH(emb1), mMYH(L1) and mMYH(L2), were localized on the chromosomes. In vivo promoter assay using the gene encoding green or red fluorescent protein and linked to the 5'-flanking region of mMYH demonstrated that the transcripts of fast-type mMYH(emb1), first expressed in embryos but belonging to the adult type in phylogenetic analysis, were located in the horizontal myoseptum. On the other hand, embryonic fast-type mMYH(L1) and mMYH(L2) were expressed in the whole myotomes. Interestingly, cells expressing mMYH(emb1) were localized together with engrailed, and cyclopamine, which blocks hedgehog signaling, inhibited mMYH(emb1) expression as well as the formation of the horizontal myoseptum, suggesting that muscle pioneer cells express mMYH(emb1) as a key protein in the formation of the horizontal myoseptum.