Abstract
In this study, affinity purification with magnetic beads and matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis was used to screen candidate biomarkers of ovarian carcinoma. Mass spectrometry compiled from serum of 30 cases of ovarian carcinomas was compared with that from serum of 10 cases of benign group and13 cases of normal group. Interesting peaks were further sieved from ascites and cystic fluid. Peptides were deduced by searching in http://us.expasy.org/tools/tagident.html and were detected by immunohistochemistry and enzyme-linked immunosorbent assay. Results showed that two peaks of 2881 and 2897Da elevated in the serum, malignant ascite and cyst fluid of cancer groupwhen compared with normal and benign groups were deduced as gastrin-releasing peptide which was higher in the malignant tissues and serum than other two groups. It was concluded that gastrin-releasing peptide in serum might be a potential marker for ovarian carcinoma. Key words: Gastrin-releasing peptide, matrix-assisted laser desorption/ionization time of flight mass spectrometry, magnetic bead, and ovarian carcinoma.
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