Abstract
Changes in the patterns of gene expression were analyzed in ultraviolet-B (UV-B; 280–320 nm) -treated seedlings of European beech by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) of mRNA. Utilizing 12 different downstream primers and 27 degenerated 10-mer upstream primers 50 induced and 29 repressed cDNA bands were detected. Nineteen bands were subcloned and sequenced. Two clones revealed significant homologies to known database sequences: putative nascent polypeptide-associated complex alpha and 60S ribosomal protein L37. However, only the latter was confirmed to be UV-B-induced by northern blot analysis.
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