Early Cellular Responses of Osteoblast-Like Cells on Acid-Etching and Alkaline Treated Titanium

Abstract

The effects of four surface modification (acid-etching, alkaline treatment, acid-etching /alkaline treatment, and sandblasting) of commercially pure titanium (cpTi) on the early cellular responses of osteoblast-like MC3T3-E1 cells were investigated. MTT assay was used to measure the levels of cell attachment to the different surface specimens after 1-, 2-, and 3-hr cell incubation. All data were submitted to two-way analysis of variance. Cell morphology was observed by scanning electron microscopy (SEM). Results showed that initial adhesion of osteoblast-like cells was independent on the surface of cpTi modified with different method.