Abstract

Studies were carried out to demonstrate the time—course for the prolactin stimulation of uridine incorporation into RNA in mammary gland explants obtained from 12– or 13–day pregnant Balb C mice. After preincubating tissues for 1, 2 or 3 days in medium containing insulin and hydrocortisone, it was discovered that a 3–4 hr incubation period with prolactin was required before a stimulation of uridine incorporation into RNA was demonstrable. Several experiments were carried out which indicated that the apparent delayed effect of prolactin on RNA synthesis is not due to the time required for the hormone to become distributed within the tissues. In one experiment explants were preincubated with prolactin for 24 hr at 0 C. This preincubation period was used in order for prolactin to become distributed within the tissues under conditions where metabolism was suppressed. After the 0 C preincubation the time—course for the effect of prolactin on RNA synthesis at 37 C was determined. Since the delayed effect of pro...

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