Ear secondary traits related to aflatoxin accumulation in commercial maize hybrids under artificial field inoculation

Abstract

Aims of the research were: (1) to evaluate and compare 24 maize hybrids for Aspergillus flavus resistance and for aflatoxin accumulation under artificial inoculation in field experiments grown during 2005 and 2006; (2) to estimate the relationship of aflatoxin concentration with ear secondary traits. Primary ears were inoculated with a fresh spore suspension (mixture of five A. flavus isolates from Northern Italy), by spraying silks, as a modification of the non-wounding silk channel inoculation technique (SCIA); controls were both non-inoculated and sterile water-inoculated ears. Ear secondary traits, such as silk channel length measured at pollination and husk coverage at maturity, were recorded for each hybrid. The severity of ear A. flavus attack was estimated using rating scales based on the percentage of kernels with visible symptoms of infection. The aflatoxin concentration in the inoculated ears resulted, during both years, higher than in the controls; this indication confirmed that the A. flavus isolates used for the inoculum procedure were successful in accumulating mycotoxin in grains. Variability was found among the hybrids under study: aflatoxin accumulation, after artificial inoculation, ranged from 0.13 to 705.25 ng/g. The data herein presented supported the implication of two ear secondary traits in determining aflatoxin accumulation. Silk channel length recorded at pollination was negatively correlated (r = -0.54; P<0.05) with aflatoxin accumulation; on the contrary, a positive correlation (r = 0.48; P<0.05) between husk coverage rating at maturity and aflatoxin concentration suggested that a looser husk coverage is associated with higher aflatoxin accumulation. The correlation between the two mentioned ear-related traits was negative (r = -0.73; P<0.05); this indicated that hybrids showing a good coverage at pollination stage, are favoured in keeping the ear tip covered until maturity, reducing the risk of aflatoxin accumulation.