Abstract
Cell therapy for ischemic diseases by using autologous progenitor cells has been limited by the functional impairment of the cells, such as insufficient paracrine and angiogenic effects, owing to the presence of risk factors, including old age. Optimization of ultrasonic microbubble transfection (UMT) to increase the product of transferred gene in the progenitor cells is a potential strategy to improve the function of progenitor cells for clinical application. Therefore, we explored whether UMT has a beneficial effect on the angiogenic activity of aged endothelial progenitor cells (EPCs).
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