コイの脂質過酸化物の吸収におよぼすビタミンEおよびエトキシキンの影響について

Abstract

The effect of antioxidants on the absorption of lipid hydroperoxide in carp, Cyprinus carpio has been studied. The carp were divided into four groups. Group 3 was given synthetic diet with vitamin E (dl-α-tocopheryl acetate), Group 4 the diet with ethoxyquin (6-ethoxy-1, 2-dihydro-2, 2, 4-trimethylquinoline) and the others synthetic diet only. After they were fed on these diets for two weeks, the following 14C-labelled compounds were administered by polyethylene catheter. Group 1: 1-14C-methyl linoleate (1-14C-ML) Group 2: purified 1-14C-methyl linoleate hydroperoxide, POV 5960meq/kg (1-14C-MLHP) Group 3: 1-14C-MLHP plus vitamin E Group 4: 1-14C-MLHP plus ethoxyquin After 24 hours, all the fish were killed. The distribution of radioactivity in various tissues was compared among the four groups. The results are summarized as follows: 1) The carp absorbed little hydroperoxide of methyl linoleate. 2) The distribution of radioactivity in Group 3 was about the same as that in the control (Group 1). It is suggested therefore that vitamin E may act as a peroxide decomposer and stabilizing factor of intestinal epithelium. 3) Since the radioactivity in Group 4 was negligible, ethoxyquin was not effective for absorption of hydroperoxide in vivo.