E. coli Gene Expression Protocols

Abstract

Cold-Inducible Promoters for Heterologous Protein Expression Francois Baneyx and Mirna Mujacic Dual-Expression Vectors for Efficient Protein Expression in Both E. coli and Mammalian Cells Rebecca L. Mullinax, David T. Wong, Heidi A. Davis, Kerstein A. Padgett, and Joseph A. Sorge A Dual-Expression Vector Allowing Expression in E. coli and P. pastoris, Including New Modifications Angelika Lueking, Sabine Horn, Hans Lehrach, and Dolores J. Cahill Purification of Recombinant Proteins from E. coli by Engineered Inteins Ming-Qun Xu and Thomas C. Evans, Jr Calmodulin as an Affinity Purification Tag Samu Melkko and Dario Neri Calmodulin-Binding Peptide as a Removable Affinity Tag for Protein Purification Wolfgang Klein Maltose-Binding Protein as a Solubility Enhancer Jeffrey D. Fox and David S. Waugh Thioredoxin and Related Proteins as Multifunctional Fusion Tags for Soluble Expression in E. coli Edward R. LaVallie, Elizabeth A. DiBlasio-Smith, Lisa A. Collins-Racie, Zhijian Lu, and John M. McCoy Discovery of New Fusion Protein Systems Designed to Enhance Solubility in E. coli Gregory D. Davis and Roger G. Harrison Assessment of Protein Folding/Solubility in Live Cells Rhesa D. Stidham, W. Christian Wigley, John F. Hunt, and Philip J. Thomas Improving Heterologous Protein Folding via Molecular Chaperone and Foldase Co-Expression Francois Baneyx and Joanne L. Palumbo High-Throughput Purification of PolyHis-Tagged Recombinant Fusion Proteins Thomas Lanio, Albert Jeltsch, and Alfred Pingoud Co-Expression of Proteins in E. coli Using Dual Expression Vectors Karen Johnston and Ronen Marmorstein Small-Molecule Affinity-Based Matrices for Rapid Protein Purification Karin A. Hughes and Jean P. Wiley Use of tRNA-Supplemented Host Strains for Expression ofHeterologous Genes in E. coli Carsten-Peter Carstens Screening Peptide/Protein Libraries Fused to the l Repressor DNA-Binding Domain in E. coli Cells Leonardo Marino-Ramirez, Lisa Campbell, and James C. Hu Studying Protein-Protein Interactions Using a Bacterial Two-Hybrid System Simon L. Dove Using Bio-Panning of FLITRX Peptide Libraries Displayed on E. coli Cell Surface to Study Protein-Protein Interactions Zhijian Lu, Edward R. LaVallie, and John M. McCoy Use of Inteins for the In Vivo Production of Stable Cyclic Peptide Libraries in E. coli Ernesto Abel-Santos, Charles P. Scott, and Stephen J. Benkovic Hyperphage: Improving Antibody Presentation in Phage Display Olaf Broders, Frank Breitling, and Stefan Dubel Combinatorial Biosynthesis of Novel Carotenoids in E. coli Gerhard Sandmann Using Transcriptional-Based Systems for In Vivo Enzyme Screening Steven M. Firestine, Frank Salinas, and Stephen J. Benkovic Identification of Genes Encoding Secreted Proteins Using Mini-OphoA Mutagenesis Mary N. Burtnick, Paul J. Brett, and Donald E. Woods Index