( E)4-[4- N, N-dimethylaminophenyl]but-3-en-2-one (DMAP) treatment inhibits the radiation-induced micronucleus formation in bone marrow of BALB/c mice

Abstract

The frequency of micronucleated polychromatic (MPCE) and normochromatic erythrocytes (MNCE) and polychromatic/normochromatic erythrocyte ratio (P/N ratio) was studied in the bone marrow of BALB/c mice administered with 20 mg/kg body weight of ( E)4-[4- N, N-dimethylaminophenyl]but-3-en-2-one (DMAP), a phenyl-butenone derivative, 30 min before exposure to different doses of 60Co γ-radiation. A dose-dependent increase in the frequency of MPCE and MNCE was observed in double-distilled water (DDW) or oil + irradiated and DMAP + irradiated groups. The frequency of MPCE and MNCE was significantly less in the DMAP + irradiated group when compared to the DDW or oil + irradiated groups at all the irradiation doses studied. The P/N ratio declined with increasing radiation dose in DDW or oil + irradiated and DMAP + irradiated groups. The inhibitory effect of irradiation on the P/N ratio was less in the DMAP + irradiated group as compared to the DDW or oil + irradiated groups, as evidence by the higher P/N ratio in the former than the latter. The P/N ratio was significantly higher in the DMAP + irradiated group from 1 Gy irradiation onwards. The dose-response relationship was linear-quadratic for MPCE, MNCE and P/N ratio for all the groups studied.