Abstract
BackgroundHepatocellular carcinoma (HCC) is one of the most common global malignancies with increasing morbidity and mortality. The purpose of this study was to investigate the expression levels and prognostic value of microRNA-3607 (miR-3607) in patients with HCC.MethodsThe expression of miR-3607 was estimated by quantitative real-time RT-PCR. Survival analysis using the Kaplan-Meier method and Cox regression analysis was conducted to evaluate the prognostic value of miR-3607. The functional role of miR-3607 in HCC progression was further assessed using gain- and loss-of-function experiments. Bioinformatics analysis and a dual-luciferase reporter assay were used to explore the direct targets of miR-3607.ResultsmiR-3607 expression was found to be significantly decreased in HCC tissues and cells compared with the matched tissues and cells (P < 0.001). The decreased expression of miR-3607 was associated with the patients’ tumor size and TNM stage (all P < 0.05). According to the survival curves, patients with low miR-3607 expression had poorer overall survival than those with high levels (log-rank P = 0.012). Moreover, the Cox analysis results indicated that miR-3607 expression was an independent prognostic factor for HCC. The results of cell experiments revealed that the overexpression of miR-3607 in HCC cells led to the inhibited cell proliferation, migration, and invasion. TGFBR1 was identified as a direct target of miR-3607.ConclusionThe data of this study indicated that the decreased expression of miR-3607 in HCC predicts poor prognosis and the overexpression of miR-3607 in HCC cells can suppress the tumor progression by targeting TGFBR1. This study provides a novel insight into the prognosis and treatment of HCC, and miR-3607 serves as a candidate prognostic biomarker and therapeutic target of HCC.
Highlights
Hepatocellular carcinoma (HCC) is a common global malignancy worldwide and one of the leading causes of cancer-related mortality [1,2,3]
Expression levels of miR-3607 in HCC tissues and cell lines In the current study, miR-3607 expression levels in the 122 paired HCC tissues and normal tissues were estimated by Quantitative real-time reverse transcriptase (RT)-PCR (qRT-PCR)
The expression results in HCC cells were consistent with the results in tissues, which showed a significant decrease in the expression of miR-3607 in HCC cell lines (Huh7, Hep3B, Li7, and SNU449) compared with the normal cells
Summary
Hepatocellular carcinoma (HCC) is a common global malignancy worldwide and one of the leading causes of cancer-related mortality [1,2,3]. The statistics indicated that the rates of HCC incidence and mortality are increasing, especially in Africa and Asia [4]. Progress has been made in therapeutic strategies, the prognosis of HCC remains not ideal, especially in advanced patients [8]. There is an urgent need for novel prognostic biomarkers that can help to predict clinical outcomes and direct timely therapy for patients with HCC. Hepatocellular carcinoma (HCC) is one of the most common global malignancies with increasing morbidity and mortality. The purpose of this study was to investigate the expression levels and prognostic value of microRNA-3607 (miR-3607) in patients with HCC
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