Abstract
BackgroundDiabetic retinopathy is a common complication of diabetes mellitus (DM). The purpose of this study was to investigate the expression and clinical significance of miR-210 in DR patients and explore the regulatory effect of miR-210 on vascular endothelial cell function under high-glucose condition.MethodsQuantitative real-time PCR was used to estimate miR-210 expression. A receiver operating characteristics curve (ROC) was plotted to evaluate the diagnostic value of miR-210. Human umbilical vein endothelial cells (HUVECs) were used and treated with high glucose (30 mM), and the cell proliferation was assessed by MTT assay.ResultsSerum expression of miR-210 was upregulated in DR patients compared with DM without DR patients and healthy controls. The expression of miR-210 in proliferative DR (PDR) patients was higher than non-proliferative DR (NPDR) patients. The increased serum miR-210 could be used to distinguish DR cases from healthy individuals and also simple DM patients, and can screen PDR cases from NPDR cases. The overexpression of miR-210 promoted HUVEC proliferation, while the knockdown of miR-210 resulted in the opposite effect under a high-glucose condition.ConclusionThe data of this study demonstrated that serum increased miR-210 serves as a diagnostic biomarker in DR patients and may have the ability to predict DR development and severity. The regulatory effect of miR-210 on vascular endothelial cell proliferation under high-glucose condition, indicating its therapeutic potential in the treatment of diabetic vascular diseases.
Highlights
Diabetic retinopathy is a common complication of diabetes mellitus (DM)
Compared with the HC group, the levels of fasting plasma glucose (FPG), Glycosylated hemoglobin (HbA1c), and Homoeostasis model assessment of insulin resistance (HOMA-IR) were significantly increased in the NDP, non-proliferative Diabetic retinopathy (DR) (NPDR), and proliferative DR (PDR) groups, and show a gradually increasing trend form the NDP group to the NPDR group to the PDR group
The levels of disease course, FPG and HbA1c were significantly higher in both NPDR, and PDR groups than that in NDR group
Summary
Diabetic retinopathy is a common complication of diabetes mellitus (DM). The purpose of this study was to investigate the expression and clinical significance of miR-210 in DR patients and explore the regulatory effect of miR-210 on vascular endothelial cell function under high-glucose condition. Diabetic retinopathy (DR) is one of the most frequent microvascular complications of diabetes mellitus (DM) [1]. It is considered a leading cause of blindness in the working-age adult, leading to a huge economic and social burden [2]. The identification of molecules involving the regulation of vascular endothelial cell function and angiogenesis may help to improve the treatment of DR. Dysregulation of miR-210 is involved in the progression of DM [12], and its promoting effect on angiogenesis by increasing vascular endothelial cell proliferation has been reported in several previous studies [13, 14]. The expression data of miR-210 in DR has not been investigated
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