Dysregulated pathways for off-pump coronary artery bypass grafting

Abstract

AbstractBackgroundThe objective of this paper was to identify dysregulated myocardial pathways with off-pump coronary artery bypass grafting (OPCABG) based on pathway interaction network (PIN).MethodologyTo achieve this goal, firstly, gene expression profiles, protein-protein interactions (PPIs) and pathway data were collected. Secondly, we constructed a PIN by integrating these data and Pearson correlation coefficient (PCC) algorithm. Next, for every pathway in the PIN, its activity was counted dependent on the principal component analysis (PCA) method to select the seed pathway. Ultimately, a minimum pathway set (MPS) was extracted from the PIN on the basis of the seed pathway and the area under the receiver operating characteristics curve (AUROC) index, and pathways in the MPS were denoted as dysregulated pathways.ResultsThe PIN had 1,189 nodes and 22,756 interactions, of which mitochondrial translation termination was the seed pathway. Starting with mitochondrial translation termination, a MPS (AUROC = 0.983) with 7 nodes and 26 edges was obtained. The 7 pathways were regarded as dysregulated myocardial pathways with OPCABG.ConclusionThe findings might provide potential biomarkers to diagnose early, serve as the evidence to perform the OPCABG and predict inflammatory response and myocardial reperfusion injury after OPCABG in the future.