Abstract
G protein-coupled receptors (GPCRs) belong to the cell membrane protein family, which regulate various physiological process such as reproduction, behavior and immune etc. In order to identify GPCRs in pheromone gland of Maruca vitrata, we carried out transcriptome analysis from virgin female adults. Fifty GPCR genes were identified such as pheromone biosynthesis activating neuropeptide receptor, prostaglandin receptors, neuropeptide receptor, 5-hydroxytryptamine receptor, galanin receptor, calcitonin gene-related peptide receptor, diuretic hormone receptor, gonadotropin-releasing hormone receptor, frizzled and orphan receptor, etc. Expressions of the identified GPCRs were confirmed with semi-quantitative RT-PCR. Expressions of various GPCRs in the pheromone gland indicate the biological roles of pheromone gland may not be limited to the pheromone production.
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