Abstract
Background and purposeDysregulated miRNAs play an important role in many malignant tumors. However, elucidating the roles of miRNAs in cancer biology, especially in epithelial cancers, remains an ongoing process. In this study, we identified the differentially expressed miR-645 in the progressing of head and neck squamous cell carcinoma (HNSCC) and investigated its biological function.MethodsThe association between clinicopathological parameters and the expression levels of the candidated miRNAs were analyzed by using the Kaplan–Meier survival analysis. The cell growth, invasion and migration potential, and clone formation were observed to detect the functions of the miRNAs in HNSCC cells.ResultsIn the 34 HNSCC tissues with lymph node metastasis, the expression level of miR-645 was 0.54 ± 0.12, and the expression level was 0.22 ± 0.05 in the 28 tissues with non lymph node metastasis (p = 0.017). In patients with HNSCC, higher level of miR-645 expression significantly correlates with worse overall survival (p = 0.04). Ectopic expression of miR-645 promoted cell invasion and migration.ConclusionsmiR-645 play a key role in cell invasion and metastasis and their expression correlates with overall survival in the patients with HNSCC.
Highlights
Background and purposeDysregulated miRNAs play an important role in many malignant tumors
We examined the expression of miR-645 in head and neck squamous cell carcinoma (HNSCC) samples by gene chips and further confirmed in HNSCC samples and HNSCC cell lines using real-time PCR
We found that miR-645 levels were up-regulated in HNSCC tissues and highly invasive cell lines
Summary
Dysregulated miRNAs play an important role in many malignant tumors. MicroRNAs (miRNAs) are endogenous RNAs that play important gene-regulatory roles in animals via sequencespecific interactions with the 3′UTR of cognate mRNA targets, causing suppression of translation and mRNA decay [1, 2]. Subsets of miRNAs have been identified as potential diagnostic and prognostic markers in malignant tumors [6,7,8]. Many evidences suggest that the regulatory capacity of miRNAs is dysregulated and exploited in malignant tumors [9]. Several miRNAs are up-regulated in specific tumors appears a general trait of human cancers which playing a causal role in the transformed phenotype [10,11,12], and the mechanisms are remain to be known further. The number of verified human miRNAs is still expanding, the functions of only a few of them have been described
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