Abstract
This study aimed to investigate the correlation between microRNA (miR)-4429 and epidermal growth factor receptor (EGFR), the expression, and clinical significance of miR-4429 in patients with non-small cell lung cancer (NSCLC), and the relationship between miR-4429 and EGFR mutation in NSCLC patients. Blood samples were collected from 122 NSCLC patients and 72 healthy volunteers. miR-4429 expression and EGFR mRNA expression were detected by real-time quantitative polymerase chain reaction. Correlation between miR-4429 and EGFR was evaluated by dual-luciferase reporter assay and the Pearson correlation analysis. The ability of serum miR4429 to discriminate between NSCLC patients and healthy controls, and to discriminate between EGFR wild-type (EGFR-W) and EGFR mutant-type (EGFR-M) patients was assessed using receiver operating characteristic analysis. The relationship between miR-4429 and NSCLC patients’ survival was identified by KaplanMeier survival curves and log-rank test. The prognostic value of miR-4429 in NSCLC patients was evaluated by Cox regression analysis. miR-4429 could directly bind to EGFR. Serum miR-4429, decreased in NSCLC patients, was negatively correlated with serum EGFR mRNA expression in NSCLC patients. In addition, miR-4429 had a high diagnostic value for screening NSCLC patients from healthy controls, and was independently correlated with survival prognosis of NSCLC patients. Moreover, miR4429 was decreased in EGFR-M patients, which had a certain screening ability for EGFRM patients. Our findings indicate that miR-4429 is negatively correlated with EGFR in NSCLC, and may function as a diagnostic and prognostic biomarker for NSCLC patients. Additionally, miR-4429 is associated with EGFR mutation in NSCLC patients.
Highlights
Lung cancer is a malignant tumor with high morbidity and mortality [1]
Circulating miR-4429 is negatively correlated with epidermal growth factor receptor (EGFR) in Non-small cell lung cancer (NSCLC) patients
The luciferase reporter assay results demonstrated that relative luciferase activity of the WT-EGFR group was decreased by miR-4429 mimic in HEK293T cells (p < 0.05), and no significant change was found in the luciferase activity of the MUT-EGFR group, indicating the direct binding between EGFR and miR-4429 (Figure 1B)
Summary
Lung cancer is a malignant tumor with high morbidity and mortality [1]. Non-small cell lung cancer (NSCLC) is the major subtype of lung cancer, accounting for 85% of lung cancer cases [2]. Despite current advances in diagnostic and therapeutic approaches, the prognosis of lung cancer remains poor with very low survival rates [3-5]. Early diagnosis and accurate prognosis are urgent issues in the treatment of NSCLC. Submitted: 01 September 2021/Accepted: 05 January 2022/ Published online: 15 February 2022
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