Abstract
Dysmorphogenic effects of di- n-butyltin dichloride (DBTC) were evaluated in vitro using the rat whole embryo culture system. Wistar rat embryos were explanted on day 8 of gestation and cultured for 68 hr. DBTC was added to the culture medium at the start of culture to achieve final concentrations of 3, 10 and 30 ng/ml. At the end of culture, the embryos were examined for vascularization development in the body and yolk sac, yolk sac diameter, crown-rump length, number of somite pairs, morphological score and external anomalies. Significantly and markedly decreased incidences of embryos with well developed vascularization in the body and yolk sac, yolk sac diameter, crown-rump length and number of somite pairs were found at 30 ng/ml. A concentration-dependent decrease in the morphological score and increase in the incidence of embryos with anomalies were noted, and the differences were significant for embryos exposed to 10 and 30 ng/ml DBTC. Open anterior neuropore and craniofacial abnormality were predominantly observed. It is concluded that DBTC possesses dysmorphogenic effects on postimplantation embryos in vitro.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
