Abstract
Lymphocytes, the cellular effectors of adaptive immunity, are involved in the chronic inflammatory process known as atherosclerosis. Proatherogenic and atheroprotective properties have been ascribed to B cells. However, information regarding the role of B cells during atherosclerosis is scarce. Both the frequency and the phenotype of B cell subpopulations were studied by flow cytometry in wild type and apolipoprotein-E-deficient (apoE−/−) mice fed a high-fat (HFD) or control diet. Whereas the proportion of follicular cells was decreased, transitional 1-like cells were increased in mice with advanced atherosclerotic lesions (apoE−/− HFD). B cells in atherosclerotic mice were more activated, indicated by their higher surface expression of CD80, CD86, CD40 and CD95 and increased serum IgG1 levels. In the aorta, a decreased frequency of B cells was observed in mice with advanced atherosclerosis. Low expression of CD19 was observed on B cells from the spleen, aorta and lymph nodes of apoE−/− HFD mice. This alteration correlated with serum levels of IgG1 and cholesterol. A reduction in CD19 expression was induced in splenic cells from young apoE−/− mice cultured with lipemic serum. These results show that mice with advanced atherosclerosis display a variety of alterations in the frequency and phenotype of B lymphocytes, most of which are associated with dyslipidemia.
Highlights
Data in support of dyslipidemia-associated alterations in B cell subpopulations frequency and phenotype during experimental atherosclerosis
Rincón-Arévalo et al / Data in Brief 7 (2016) 958–972 data contained in this article shows and supports that mice with advanced atherosclerosis have a variety of alterations in frequency and phenotype of B cell subsets, most of which associated with dyslipidemia. & 2016 The Authors
Data Here we describe detailed materials and methods, the analysis strategy for discriminating B cells from aortas, lymph nodes and spleens and some aspects of the phenotype of B cell subsets in the atherosclerosis mouse model apoE-/- and control mice (Figs. 1–14)
Summary
Mice were euthanatized with CO2 exposition and heart, spleen, lymph nodes and aorta were harvested by dissection. Hearts were embedded in paraformaldehyde solution (4%, JT Baker, NJ) at 4 °C during 48 h, and in sucrose solution (30%, Sigma-Aldrich, MO) for 24 h. Heart tissues were stored at À 20 °C in Shandon cryomatrix (Thermo Scientific, PE) until their use. Aortas and lymph nodes were collected in phosphate buffered saline (PBS, Gibco, NY) at 4 °C and immediately processed. Peripheral blood obtained by cardiac puncture was centrifuged at 10.000 Â g during 10 min at 4 °C to obtain serum. Serum was stored at À 20 °C until used. Heart tissues were serially sectioned in a CM-1850 Cryostat (Leica Microsystems, Germany) for 6–7 mm thickness on slides with positive charge (Thermo scientific), and stained with Hematoxylin–. Images were analyzed with NIS Element Software (Nikon) to determinate the size of atherosclerotic lesions
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
