Abstract
Based on our hypothesis for existing microbiota of wall-deficient variants (L-forms) in human blood, we created an innovative methodology, which allowed for the development of L-form populations from blood of all investigated people. In contrast to healthy controls, blood L-forms from autistic children and their mothers converted under appropriate conditions of cultivation into detectable opportunistic bacteria and fungi, а process demonstrated by light and transmission electron microscopy. It can be distinguished into two types of states – “eubiotic” blood microbiota in healthy individuals, and “dysbiotic” in autistic children and their mothers. Remarkably, the unifying finding for autistic children and their mothers was the presence in blood of wall-free variants from life-cycle of filamentous fungi. Increased specific IgG, IgM and IgA, together with typical mold growth were a decisive argument for proven presence of Aspergillus fumigatus in almost all of the autistic children. As it was demonstrated in our previous study, filterable L-forms can be transmitted by vertical pathway from mother to child before birth. Thus, it can be suggested that autistic children may be born already colonized with fungi, while a “silent aspergillosis” could contribute or even be a leading cause for neurodevelopmental disorders in the early childhood.
Highlights
Based on our hypothesis for existing microbiota of wall-deficient variants (L-forms) in human blood, we created an innovative methodology, which allowed for the development of L-form populations from blood of all investigated people
On the basis of accumulated knowledge about the unique biology/ nature of cell wall deficient microbes, we have developed an innovative methodology for evaluation of human blood microbiota, which would be of importance for diagnosis of hidden latent infections[3,4,5]
The accumulated knowledge of microbial cell wall-deficient variants, or the so called L-form phenomenon, gives reason to believe that a community from L-forms could constitute a microbiota in the human blood[3,4,5,6]
Summary
Based on our hypothesis for existing microbiota of wall-deficient variants (L-forms) in human blood, we created an innovative methodology, which allowed for the development of L-form populations from blood of all investigated people. In contrast to healthy controls, blood L-forms from autistic children and their mothers converted under appropriate conditions of cultivation into detectable opportunistic bacteria and fungi, а process demonstrated by light and transmission electron microscopy. Systemic microbial persistence can be suspected to be a contributing factor to autism but the causing microbes are difficultly proven with conventional approaches. That is why their presence and significance have often been questioned. On the basis of accumulated knowledge about the unique biology/ nature of cell wall deficient microbes, we have developed an innovative methodology for evaluation of human blood microbiota, which would be of importance for diagnosis of hidden latent infections[3,4,5]. The goal of the current study was to isolate cell wall deficient variants (L-forms) from blood of autistic children, their mothers and control healthy persons, to observe and analyze their morphological transformations and characteristics, as well as to identify them after recovering of their cell walls
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