Abstract
The dynamics of the co-ordinating water and metal cations in solid hydrates of nucleotide salts is explored with solid-state NMR spectroscopy and DFT calculations.
Highlights
A thorough description of the structure of the solvent shells surrounding nucleic acid (NA) systems is important for understanding most molecular recognition processes, ranging from sequence-specific DNA recognition[1,2,3,4] to drug– nucleic acid interactions[5,6,7] and DNA conduction
The content of crystal water molecules was determined by elemental analysis (ESI†) and it differed only slightly, in the range 6.5–7 water molecules per nucleotide, depending on the crystallisation conditions. 13C CP magic-angle spinning (MAS) spectra of the uridine monophosphate system (UMP) samples recrystallized from different solvent mixtures were virtually identical
Two signals corresponding to the two non-equivalent molecules of guanosine monophosphate system (GMP) are present in the 31P NMR spectra in the temperature range −40 to 40 °C together with a third signal, which corresponds to a dehydrated form (Fig. 3)
Summary
A thorough description of the structure of the solvent shells surrounding nucleic acid (NA) systems is important for understanding most molecular recognition processes, ranging from sequence-specific DNA recognition[1,2,3,4] to drug– nucleic acid interactions[5,6,7] and DNA conduction. Metal ions are involved in almost every aspect of nucleic acid chemistry, ranging from neutralization of the anionic nucleic acids[12] through specific stabilization of three-dimensional structures of NA molecules up to their effect as cofactors in RNA-mediated catalysis.[13]. The solvation shell dynamics are difficult to assess by these techniques
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.