Dynamics of ultrastuctural changes in the secretory apparatus of thyrocytes in the conditions of an experimental blastomogenesis.

Abstract

Background. Secretion of thyroid hormones considerably decreases at development of tumoral pathology of various localization and significantly affects efficiency of treatment and the forecast of a disease. Development of a hypothyroid state can be connected with disturbance of secretory activity thyrocytes of a thyroid gland and contributes to the development of system thyroid deficiency. Definition of structural bases of development of thyroid deficiency at tumoral pathology at the subcellular level in distance interaction of a thyroid gland and a tumor will allow to define molecular and cellular mechanisms of interaction of an organism and a tumor in the a blastomogenesis. Objective. The purpose of the research was studying of a state and dynamics of changes of a lamellar complex of thyrocytes in the course of distance interaction with a tumor in an experiment. Methods. As the experimental model of tumor we have used a model of the slightly differentiated solid adenocarcinoma of Guerin. Experiment carried out on the rats - the males of the line of Vistar as far as the mass 120 - 160 g. animals were divided beside 2 groups. First group - control. To the second group - translanted the strain of the cells of Guerin's carcinoma by hypodermic injection beside the region thighs. Animals derived based on the experiment on 2, 4 and 12 weeks. Cytotopographical and ultramicroscopic studying of dynamics of changes of dictyosomes, their distribution in cytoplasm of thyrocytes and a condition of structural components of a lamellar complex is carried out. Results. On the 2nd week of an experimental blastomogenesis reduction of number of cisterns of a lamellar complex to 3 - 4 is observed, cis - cisterns are considerably expanded, distances between cisterns are increased when comparing with control group, length of cisterns of trans - compartment is reduced when comparing with control group. On the 4th week of growth of a transplanted tumor the significant changes of a lamellar complex connected with fragmentation of cisterns, loss of polarity of dictyosomes and lack of vesicles and vacuoles around a lamellar complex are noted. On the 12th week of growth of a carcinoma of Guerin in thyrocytes reduction of length of cisterns of a lamellar complex, their mutually parallel and dense arrangement, lack of ampullar expansions, insignificant increase in volume of cisterns of trans - compartment dictyosome is observed. Conclusion. Changes of ultrastructure of a lamellar complex of Golgi of thyricytes of a thyroid gland in the course of distance interaction with the transplanted tumor demonstrate early disturbance of processes of secretion and vesicular transport with possible development of apoptosis due to activation of a caspase system.