Dynamics of TNF-α and INF-γ content in blood serum in connection with the marker profile of patients with acute hepatitis B at different degrees of severity of the disease

Abstract

The aim – is to analyze the dynamics of the content of TNF-α and INF-γ in the serum in connection with the marker profile of patients with acute hepatitis B at different degrees of severity of the disease. Materials and methods. 32 patients with AHB were examined in the dynamics of the disease with the determination of HBsAg, HBeAg, anti-HBs and anti-HBe, the content of TNF-α and IFN-γ by ELISA. Statistical processing was performed in Statistica 13 for Windows (StatSoft Inc., No. JPZ804I382130ARCN10-J). Results. Patients with severe (40.6 %) and moderate (37.5 %) courses of the disease predominate among patients with AHB. Severe course of AHB, compared with the moderate course, was characterized by higher levels of hyperbilirubinemia (P < 0.05), cytolytic syndrome (P < 0.05), hypoproteinemia (P < 0.05), and signs of hypocoagulation (P < 0.05). HBsAg (P < 0.05) was more often observed in patients with moderate and severe AHB (P < 0.05) in the absence of a difference in the frequency of seroconversion for HBeAg. Seroconversion of HBsAg/anti-HBs at 6 months was lowest after mild (66.7 %), compared with moderate (91.7 %) and severe (100.0 %) (P > 0.05) course of the disease. Regardless of the severity of AHB, the content of TNF-α was higher (P < 0.05) than in healthy individuals and highest in the severe course of the disease (P < 0.05). The content of INF-γ was higher than in healthy individuals (P < 0.05), regardless of the severity of the disease. TNF-α correlated with the severity of AHB (r = 0.57, P < 0.05), the level of total bilirubin (r = 0.65, P < 0.05), INR (r = 0.42, P < 0.05) and PI (r = -0.48, P < 0.05) at hospitalization. Conclusions. Patients with severe (40.6 %) and moderate (37.5 %) courses of the disease predominate among the hospitalized patients with AHB. At hospitalization, the content of TNF-α is highest in the severe course of the disease (P < 0.05). The content of INF-γ is higher than in healthy individuals (P < 0.05), regardless of the severity of the course of AHB. At hospitalization, all patients were positive for HBsAg and IgM anti-HBcorAg, the vast majority of patients for HBeAg. Positive HBsAg was more often (P < 0.05) in patients with a moderate course of AHB during discharge from the hospital. HBeAg was preserved in 28.6 % of patients with the mild course. Chronic HBV infection was observed in 3.6 % of patients with the mild course.