Dynamics of the antioxidant-prooxidant balance of the kidney under conditions of acute blood loss of various degrees and its correction

Abstract

The purpose of the study is to find out the dynamics of the antioxidant-prooxidant balance of the kidney during acute blood loss of various degrees and to evaluate the effectiveness of correction with Ringer’s lactate solution in combination with 2-ethyl-6-methyl-3-oxypyridine succinate.Material and methods. 126 male Wistar line rats weighing 0.16-0.18 kg were randomly selected for the experiment. Four experimental groups and one control group were selected. Acute blood loss of 1% of body weight was simulated in experimental group 1, acute blood loss of 2% of body weight was simulated in experimental group 2. In experimental group 3, acute blood loss in the amount of 2% of body weight was simulated, and after 1 hour, Ringer’s lactate solution was injected once into the adjacent vein in a ratio of 1:1 in the amount of blood loss volume. In experimental group 4, after simulating acute blood loss in the amount of 2% of body weight, Ringer’s lactate solution in combination with 2-ethyl-6-methyl-3-hydroxypyridine succinate at a dose of 100 mg·kg-1 was injected into an adjacent vein once. A control group of rats was only injected with thiopental sodium anesthesia. After 1, 3, and 7 days, catalase activity, the content of reagents to thiobarbituric acid were determined in the kidney, and their ratio - the antioxidant-prooxidant index (API) was calculated.Results and discussion. It was established that acute blood loss within 1-7 days of the experiment is accompanied by the accumulation of thiobarbituric acid reagents in the kidney and by a decrease in catalase activity and the value of API. Disturbances depend on the volume of blood loss and are significantly greater under conditions of acute blood loss in the amount of 2% of the mass. The injection for correction with Ringer’s lactate solution in combination with 2-ethyl-6-methyl-3-oxypyridine succinate compared to monotherapy with Ringer’s lactate solution leads to a significant decrease in the content of reagents to thiobarbituric acid in the kidney, and an increase in catalase activity and the value of API, starting from the 3-rd day of the experiment.