Dynamics of phosphorus speciation and the phoD phosphatase gene community in the rhizosphere and bulk soil along an estuarine freshwater-oligohaline gradient

Abstract

Estuarine tidal marshes play a key role in phosphorus (P) retention and cycling; however, they are suffering from small but significant increases in tidal saltwater intrusion. The likely impacts of these low-level saltwater intrusions on P availability and microbial activity are unclear. Here, we investigated soil P speciation, alkaline phosphatase (ALP) activity, and the phoD phosphatase gene community along a freshwater-oligohaline gradient in the Min River estuary, southeast China. The results indicated that with the transition from freshwater to oligohaline water, the levels of soil-water salinity, pH and sulfate content were greater, and ALP activity was lower, which were associated with higher concentrations of organic P, available P, aluminum-bound P, calcium-bound P, and occluded P and lower levels of iron-bound P. There was a strong shift in the phoD phosphatase community composition in response to the freshwater-oligohaline gradient. Our findings showed that with the transition from freshwater to an oligohaline environment, in addition to the associated increases in salinity and soil pH and decreases in general microbial and biological activity and soil organic carbon, there is a shift in soil P toward more recalcitrant and immediately available fractions with less labile forms.