Dynamics of Microbial Community and Functional Genes during Bioremediation of PAHs-Contaminated Soil by Two Biostimulants

Abstract

Biostimulation is a favored approach in bioremediation strategies for polycyclic aromatic hydrocarbons (PAHs). However, the addition of some biostimulants can instead be counterproductive to the soil. In this study, rhamnolipid and leaf litter were added as aids to PAHs biodegradation in soil. Quantitative Real-time PCR (qPCR) and 16S ribosomal RNA (16S rRNA) techniques were used to investigate the structural-functional response of soil microbial communities and changes in PAHs functional genes during 82 days of the degradation process. The study reveals that rhamnolipid and leaf litter increase the degradation rate of Benzo(a)pyrene by 20.49% and 11.64%, respectively, while having little effect on the degradation rate of phenanthrene. During the degradation process, each biostimulant increases the number of different bacterial genera capable of degrading PAHs, which influences both the structure and function of the microbial community and alters microbial diversity. Additionally, functional gene abundance was enhanced during degradation and showed an inverse relationship with residual PAHs concentration in the soil. These results indicate that different biostimulants have varying effects on microorganisms. This study enhances the understanding of useing of two biostimulants to degrade soil PAHs.