Abstract
Abstract By improving the existing methods for quantifying organic acids in paddy soils, we developed a quantitative analytical method for low-molecular-weight aliphatic carboxylic acids (LACAs) in forest soils. Appropriate pre-treatment and concentration of soil extracts in combination with HPLC analysis where two types of columns and detectors were introduced enabled to obtain a sensitive determination of LACAs in forest soils. Moreover, choice of extractant enabled us to differentiate the existing forms of LACAs, namely water-soluble and adsorbed ones, the latter reflecting the difference between the phosphate buffer-soluble and water-soluble forms. By applying this method to the upper parts of the A and B horizon samples from a Podzolic soil, two Brown Forest soils and two Andosols, the following results were obtained. 1) Comparison between the upper part of the A and B horizons of each soil profile indicated that fairly comparable amounts of LACAs were present in the B horizon as well as A horizon. 2...
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