DYNAMICS OF INFLUENCE OF SOME HEAVY METALS ON THE BIOSYNTHETIC CAPACITY OF STREPTOMYCES RECEFENSIS VAR. LYTICUS 2P-15 FOR THE SYNTHESIS OF AMYLOLYTIC ENZYMES

Abstract

Modern biotechnology of enzymes is a promising and rapidly developing branch of industrial biotechnology, which requires new promising research in the aspect of optimizing the conditions of biosynthesis of enzymes. Modulation of the biosynthetic activity of producer strains allows to significantly increase the economic output of production. The most important factor in increasing efficiency is the composition of the nutrient medium, which can be optimized depending on the needs of the microorganisms. Within the framework of a complex study of the optimization of the composition of the nutrient medium by the simplex method of mathematical modeling, the aim of the work is to study the effect of some heavy metal ions on the biosynthetic capacity of the strain Streptomyces recefensis var. lyticus 2P-15. The influence of some heavy metal ions on the biosynthetic capacity of the strain Streptomyces recefensis var. lyticus 2P-15 in terms of synthesis of amylolytic enzymes, accumulation of biomass and protein. The object of the study is the strain Streptomyces recefensis var. lyticus 2P-15, obtained by three-stage selection of the producer. The simplex method of selecting the composition of the environment was used for the research. Standard methods for determining biomass and amylolytic activity were used to analyze biosynthetic activity. It was established that adding Co, Mo, and Cd ions at a concentration of 0.00001 and 0.0005 mg/ml inhibits the growth of biomass, but increases the protein content by 13-57%, respectively. It was determined that the addition of Co, Mo, Cd ions in different concentrations helps to increase both the quantitative and qualitative composition of the synthesized enzymes, and the best response was an increase in amylolytic activity by 354% compared to the control. The prospect of further research into the optimization of the biosynthesis of actinomycetes simplex by the method of other aspects of its regulation will increase the biosynthetic capacity of the studied strain, which will positively affect the economic output of the production of amylolytic enzyme preparations by obtaining microbial synthesis.