Dynamics of indices of nonspecific resistance in chicken blood following ofloxacin administration

Abstract

In the present publication we share the results of our research into the effect of ofloxacin, a fluoroquinolone class drug, on leukocyte indices of Hisex Brown male chicks. We studied the indices in 2 groups: I – control group, which received pure drinking water, II – experimental group, which received ofloxacin via drinking water at concentration 200 mg/L for 10 consecutive days. Blood samples were drawn at Day 1, Day 3, Day 5, Day 7 and Day 9 after the drug withdrawal. We performed WBC differential for all the employed chicks; then by applying computational method we studied the dynamics of Krebs index (KI), leukocyte index (LI) and index of immunoreactivity (IIR). We observed a transitory decrease in KI by 25% on Day 3 and an increase in LI by 23% on the same day. These reliable changes occurring only once show that ofloxacin did not have a marked negative effect on these indices. We also observed a shift in IIR, here the dynamics was ambiguous. Accordingly IIR increased reliably by 47% on Day 1 as compared to control, but on Day 5 and Day 7 it decreased by 41% and 54% respectively. We noted continuous dynamics in IIR on Day 1, Day 5 and Day 7 but on the final day of the experiment, at the close, there were no reliable changes and that proves, with high probability, the absence of any negative effect of ofloxacin, when administered at concentration 200 mg/L, on physiological state of Hisex Brown chickens at young age.