Dynamics of estrogenic activity in an urban river receiving wastewater effluents: effect-based measurements with CALUX

Abstract

Estrogenic substances (ES) in an urban river Zenne (BE) dominated by wastewater effluents were assessed over the course of one year. To measure the bioequivalent (BEQ) 17 β-estradiol (E2) concentrations of ES, the biological effect-based methodology - the Chemical-Activated LUciferase gene eXpression (CALUX) bioassay was used. Daily water discharges were collected from January 2015 to February 2016 at or near the sampling stations in the Brussels Capital Region. An annual water budget shows that approximately 50% of the Zenne River flow downstream is from wastewater effluent. The estrogenic activity and yearly average ES load in influents and effluents of wastewater treatment plants (WWTPs) located in the North and South, combined sewer overflows (CSOs) and the Zenne River, were assessed for upstream and downstream of two WWTPs of Brussels. Both WWTPs with activated sludge treatment remove more than 90% of the ES. The influent concentrations of ES at the South and North WWTPs ranged from 30-359 and 18-55 ng E2 eq./L, respectively. The effluent concentrations of ES ranged from 1.0-2.1 and 1.1-6.6 ng E2 eq./L at WWTP-S and -N, respectively. The yearly average ES loads were 0.05-0.14 and 0.39-1.5 g E2 eq./d for WWTP-S and -N, respectively. The temporal variation of E2-eq concentrations at the river stations Z3 and Z5 (upstream) ranged from 1 to 2 ng E2 eq./L, while the ES activity at sites Z9 and Z11 (downstream) varied from 2-17 ng E2 eq./L and from 1-8 ng/L ng E2 eq./L, respectively. The relative ES loads to the Zenne River are as follows: WWTPs (31%), CSOs (27%), upstream Zenne (15%), a missing source (14%), and local tributaries (13%). ES in the Zenne River behave in a pseudo-persistent manner because of continuous input from the WWTPs and slow degradation in the 18 km river stretch. The BEQ concentration of E2 exceeds the EU environmental quality standards (EQS) of 0.4 ng E2/L throughout the Zenne River.