Dynamics of cytokines and heat shock proteins mRNA expression in thermally manipulated chicken challenged with intraperitoneally injection of Lipopolysaccharide (LPS).

Abstract

Heat stress is known to significantly affect broiler immunity and cytokine expression, but it is not yet clear how embryonic thermal manipulation (TM) might help alleviate the aforementioned effects. The aim of the present study was to evaluate the effects of TM on the splenic mRNA expressions of IL‐1β, IL‐6, TNF‐α, IFN‐α, IFN‐β, IFN‐γ, IL‐4, IL‐8, IL‐15, IL‐16, IL‐17, IL‐18, Hsp70 and HSF‐3 in broilers intraperitoneally injected with lipopolysaccharide (LPS). TM was performed by elevating the incubation temperature to 39°C at 65% relative humidity for 18 h daily during embryonic days 8 to 16. Whereas, control group was incubated at standard condition during the whole incubation period (37.8°C and 56% relative humidity). LPS was intraperitoneally injected at a dose of 0.5 mg/kg body weight (BW) on post‐hatch days 10 and 24. spleens was collected from all groups to evaluate mRNA expression by relative‐quantitative real‐time RT‐qPCR. TM results in alteration in the mRNA expression dynamics of cytokine and heat shock protein following intraperitoneally injection of LPS. The results of this study provided us with a valuable information about the mechanisms by which thermal manipulation may modify the immune system response in the thermally manipulated chicken.Support or Funding InformationDeanship of Research/ Jordan University of Science & Technology for its financial support of this work (Grant#: 44/2019).