Dynamics and Mechanism of Laser Trapping-Induced Crystal Growth of Hen Egg White Lysozyme

Abstract

We propose the dynamics and mechanism of laser trapping-induced crystal growth of hen egg-white lysozyme (HEWL). A continuous-wave near-infrared laser beam is used as a trapping light source and focused at a point 10 μm away from a target tetragonal HEWL crystal that is spontaneously generated in solution. Laser trapping of HEWL liquid-like clusters in solution increases local concentration in the focus, where the free motion and orientation of the clusters are strongly restricted, and the clusters show high rigidity and ordering. The cluster association and reorientation at the micrometer-sized focus is evolved to a large highly concentrated domain of the clusters, where the specific target crystal is grown. Initially, the high rigidity and ordering of the clusters strongly suppress the crystal grow rate compared to spontaneous crystal growth. Continuous laser trapping at the focus of the initially formed domain, however, leads to the transition to another domain with different concentration, rigidity, a...