Dynamics and interactions of ibuprofen in cyclodextrin nanosponges by solid-state NMR spectroscopy.

Monica Ferro,Walter Panzeri,Franca Castiglione,Carlo Punta,Francesco Trotta,Lucio Melone,Nadia Pastori,Barbara Rossi,Andrea Mele

doi:10.3762/bjoc.13.21

Abstract

Two different formulations of cyclodextrin nanosponges (CDNS), obtained by polycondensation of β-cyclodextrin with ethylenediaminetetraacetic acid dianhydride (EDTAn), were treated with aqueous solutions of ibuprofen sodium salt (IbuNa) affording hydrogels that, after lyophilisation, gave two solid CDNS-drug formulations. 1H fast MAS NMR and 13C CP-MAS NMR spectra showed that IbuNa was converted in situ into its acidic and dimeric form (IbuH) after freeze-drying. 13C CP-MAS NMR spectra also indicated that the structure of the nanosponge did not undergo changes upon drug loading compared to the unloaded system. However, the 13C NMR spectra collected under variable contact time cross-polarization (VCT-CP) conditions showed that the polymeric scaffold CDNS changed significantly its dynamic regime on passing from the empty CDNS to the drug-loaded CDNS, thus showing that the drug encapsulation can be seen as the formation of a real supramolecular aggregate rather than a conglomerate of two solid components. Finally, the structural features obtained from the different solid-state NMR approaches reported matched the information from powder X-ray diffraction profiles.

Highlights

In the last ten years cyclodextrin nanosponges (CDNS) polymer materials received great attention as promising new materials in several fields of applications such as bio-catalysis, agriculture, analytical chemistry [1,2] and in pharmaceutical research
Two different formulations of cyclodextrin nanosponges (CDNS), obtained by polycondensation of β-cyclodextrin with ethylenediaminetetraacetic acid dianhydride (EDTAn), were treated with aqueous solutions of ibuprofen sodium salt (IbuNa) affording hydrogels that, after lyophilisation, gave two solid CDNS-drug formulations. 1H fast magic angle sample spinning (MAS) NMR and 13C CP-MAS NMR spectra showed that IbuNa was converted in situ into its acidic and dimeric form (IbuH) after freeze-drying. 13C CP-MAS NMR spectra indicated that the structure of the nanosponge did not undergo changes upon drug loading compared to the unloaded system
The 13C NMR spectra collected under variable contact time cross-polarization (VCT-CP) conditions showed that the polymeric scaffold CDNS changed significantly its dynamic regime on passing from the empty CDNS to the drug-loaded CDNS, showing that the drug encapsulation can be seen as the formation of a real supramolecular aggregate rather than a conglomerate of two solid components

Summary

Introduction

In the last ten years cyclodextrin nanosponges (CDNS) polymer materials received great attention as promising new materials in several fields of applications such as bio-catalysis, agriculture, analytical chemistry [1,2] and in pharmaceutical research. Their wide applicability is mostly due to their nanoporous structure, along with their high chemical and thermal stabilities. In particular the transport properties of ibuprofen sodium salt (IbuNa) entrapped in CDNS polymer gels has been investigated by high resolution magic angle spinning (HR-MAS) NMR technique [9] as a paradigmatic case of an active pharmaceutical ingredient (API) entrapped in a polymeric scaffold. Solid-state NMR spectra revealed that in all tablet samples, ibuprofen is present in acidic form IbuH with different contents of bound water within tablets [12] depending on the formulation

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