Dynamics and influencing factors of soluble organic nitrogen in paddy soil under different long-term fertilization treatments

Abstract

Soil soluble organic nitrogen (SON) plays an important role in nitrogen cycling. However, the dynamic and influencing factors of SON in paddy soil are largely unknown. In this study, 36-year fertilization experiments with different fertilized (control (CK), chemical fertilizer (NPK), chemical fertilizer combined with cow manure (NPKM) and chemical fertilizer combined with straw (NPKS)) paddy soils were selected to investigate the dynamic changes and influencing factors of the SON content during the whole rice growth stage. We found that NPK, NPKM and NPKS increased the soil SON content by 20.61 %, 51.33 % and 52.50 %, respectively, compared with CK. Compared with the NPK treatment, the average SON contents in the NPKM and NPKS treatments were significantly increased by 25.46 % and 26.44 %, respectively, but there was no significant difference between the NPKM and NPKS treatments. Soil SON contents in different growth stages under the same fertilization treatments showed maturity stage > tillering stage > flowering stage > jointing stage > seedling stage. Structural equation model (SEM) analysis showed that bacterial biomass was an important factor affecting soil SON content, with a total path coefficient of 0.92, including direct path coefficients of 0.24 for soil SON and indirect path coefficients of 0.68 for soluble organic carbon, microbial biomass nitrogen and protease activity. Redundancy sequencing analysis showed that SON contents during different rice growth stages were significantly affected by the bacterial community, i.e., Chloroflexi in the early growth stage and Proteobacteria and Bacteroidetes in the later stage contributed 5.33 %, 37.40 %, and 2.17 % to the SON content variation, respectively. Conclusively, the dynamics of SON in paddy soil under long-term fertilization treatments are closely related to both fertilization treatments and the rice growth stage and are mainly affected by the bacterial community.