Abstract
BackgroundBean common mosaic virus (BCMV) is widespread, with Phaseolus species as the primary host plants. Numerous BCMV strains have been identified on the basis of a panel of bean varieties that distinguish the pathogenicity types with respect to the viral strains. The molecular responses in Phaseolus to BCMV infection have not yet been well characterized.ResultsWe report the transcriptional responses of a widely susceptible variety of common bean (Phaseolus vulgaris L., cultivar ‘Stringless green refugee’) to two BCMV strains, in a time-course experiment. We also report the genome sequence of a previously unreported BCMV strain. The interaction with the known strain NL1-Iowa causes moderate symptoms and large transcriptional responses, and the newly identified strain (Strain 2 or S2) causes severe symptoms and moderate transcriptional responses. The transcriptional profiles of host plants infected with the two isolates are distinct, and involve numerous differences in splice forms in particular genes, and pathway specific expression patterns.ConclusionsWe identified differential host transcriptome response after infection of two different strains of Bean common mosaic virus (BCMV) in common bean (Phaseolus vulgaris L.). Virus infection initiated a suite of changes in gene expression level and patterns in the host plants. Pathways related to defense, gene regulation, metabolic processes, photosynthesis were specifically altered after virus infection. Results presented in this study can increase the understanding of host-pathogen interactions and provide resources for further investigations of the biological mechanisms in BCMV infection and defense.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-2976-8) contains supplementary material, which is available to authorized users.
Highlights
Bean common mosaic virus (BCMV) is widespread, with Phaseolus species as the primary host plants
Identification of BCMV strains utilizing a P. vulgaris bean screen Previously, the identification of NL1-I was conducted using a differential selection of bean cultivars
BCMV infection assay during common bean development Because BCMV-NL1-I and BCMV-S2 caused different reactions in susceptible host plants, we wanted to characterize the effects of these two isolates on host gene expression by using RNA-Seq analysis
Summary
Bean common mosaic virus (BCMV) is widespread, with Phaseolus species as the primary host plants. Common bean (Phaseolus vulgaris L.) is the main host of this virus, it infects other Phaseolus species [2, 3]. This virus can cause significant yield losses (50-100 %) in the host crop plants [4,5,6]. There are 8 pathogenicity groups in the BCMV complex, identified by the virus response on a standard set of differential bean lines [11,12,13]. The I locus is associated with a cluster of R gene homologs belonging to the TIR-NB-LRR
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
