Abstract
Characterization of the dynamic changes of the basic surface properties of nanoparticles is of great significance to reveal the interaction mechanism between nanoparticles and cells; however, it is often neglected due to the limitations of existing analytical methods. This knowledge has been renewed by using surface enhanced infrared absorption spectroscopy (SEIRAS) to study the interaction between PEG-CuS nanoparticles and living cells attached to rGO-Au modified Au films. Based on the difference spectra of cell membranes and the associated water, we clearly revealed that Cu2+ ions produced by the degradation of PEG-CuS can coordinate with PEG, thus changing the interaction between nanoparticles and cells including how and how many nanoparticles enter the cells and the sequential photothermal effect, which breaks through the limitations of the present analytical methods.
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