Abstract
BackgroundSpecific histone modifications play important roles in chromatin functions; i.e., activation or repression of gene transcription. This participation must occur as a dynamic process. Nevertheless, most of the histone modification maps reported to date provide only static pictures that link certain modifications with active or silenced states. This study, however, focuses on the global histone modification variation that occurs in response to the transcriptional reprogramming produced by a physiological perturbation in yeast.ResultsWe did a genome-wide chromatin immunoprecipitation analysis for eight specific histone modifications before and after saline stress. The most striking change was rapid acetylation loss in lysines 9 and 14 of H3 and in lysine 8 of H4, associated with gene repression. The genes activated by saline stress increased the acetylation levels at these same sites, but this acetylation process was quantitatively minor if compared to that of the deacetylation of repressed genes. The changes in the tri-methylation of lysines 4, 36 and 79 of H3 and the di-methylation of lysine 79 of H3 were slighter than those of acetylation. Furthermore, we produced new genome-wide maps for seven histone modifications, and we analyzed, for the first time in S. cerevisiae, the genome-wide profile of acetylation of lysine 8 of H4.ConclusionsThis research reveals that the short-term changes observed in the post-stress methylation of histones are much more moderate than those of acetylation, and that the dynamics of the acetylation state of histones during activation or repression of transcription is a much quicker process than methylation.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-247) contains supplementary material, which is available to authorized users.
Highlights
Specific histone modifications play important roles in chromatin functions; i.e., activation or repression of gene transcription
Our results indicate that the histone modifications which better correlate with transcription activity are those of H3K4me3 and H3K36me3 (Spearman correlation of 0.659 and 0.600, respectively), which is in agreement with previous reports [11,12], and that both modifications can be considered chromatin marks related with transcription elongation (Figure 3)
Our mapping data of the modifications occurring under standard growing conditions generally agree with previous studies [12], we observed several unexpected behaviors
Summary
Specific histone modifications play important roles in chromatin functions; i.e., activation or repression of gene transcription. This participation must occur as a dynamic process. Besides DNA methylation, the pattern of histone modifications has been called in the past decade support the notion that such modifications regulate transcription through the recruitment of effectors protein complexes (Reviewed in [7]). Both proposed mechanisms of action are not mutually exclusive. While there are no new genome-wide studies on the acetylation status of histones in yeast, the methylation profiles of histone H3 obtained in [12] have subsequently been corroborated by other authors using higher density chips probes [15,16,17] or ChIP-seq [18,19]
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